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KEY RESOURCES TABLE

Journal: Cell metabolism

Article Title: Microglial UCP2 Mediates Inflammation and Obesity Induced by High-Fat Feeding

doi: 10.1016/j.cmet.2019.08.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 1Lowl /J Kong et al., 2010 N/A Mouse: B6.129P2(C)- Cx3cr1 tm2.1(cre/ERT2)Jung /J Jackson Laboratory #020940 Mouse: Cx3cr1-cre:ERT2 : Ucp2 fl/fl This paper N/A Oligonucleotides Taqman probe Ucp2 Thermo Fisher Scientific Mm00627599_m1 Taqman probe IL-1 β Thermo Fisher Scientific Mm00434228_m1 Taqman probe IL-6 Thermo Fisher Scientific Mm00446190_m1 Taqman probe Tnf, Thermo Fisher Scientific Mm00443258 TaqMan Probe IL-10 Thermo Fisher Scientific Mm01288386_m1 TaqMan Probe Cx3cr1 Thermo Fisher Scientific Mm02620111_s1 TaqMan Probe Hspa1a Thermo Fisher Scientific Mm01159846_s1 TaqMan Probe Gfap Thermo Fisher Scientific Mm01253033_m1 TaqMan Probe Gapdh Thermo Fisher Scientific Mm99999915_g1 TaqMan Probe β-Actin Thermo Fisher Scientific Mm02619580 Software and Algorithms FlowJo Ver.10 Software FlowJo https://www.flowjo.com/solutions/flowjo PhenoMaster Software TSE System https://www.tse-systems.com/product-details/phenomaster ImageJ Software NIH https://imagej.nih.gov/ij/ Prism 7.01 Software GraphPad Software http://www.graphpad.com Wave 2.6.0 Software Agilent technologies https://www.agilent.com/en/products/cell-analysis/xf-cell-mito-stress-test-report-generator Other Chow Diet, 18% Calories from Fat Harlan Teklad # 2018 High Fat Die, 45% Fat Rodent Chow # D12451 Metabolic Chambers TSE System-Core Metabolic Phenotyping Center N/A LSRII BD Bioscience N/A EchoMRI Echo Medical Systems, Houston, TX N/A Dynabeads Biotin Binder Invitrogen Cat# 11047 LightCycler 480 Roche N/A All-In-One Fluorescence Microscope KEYENCE Model BZ-X710 Seahorse XF96 Analyzer Agilent Technologies https://www.agilent.com/en/products/cell-analysis/seahorse-analyzers/seahorse-xfe96-analyzer Open in a separate window KEY RESOURCES TABLE The brain plays a critical role in the regulation of feeding and whole-body metabolism.

Techniques: Plasmid Preparation, Recombinant, Expressing, Staining, Software, Fluorescence, Microscopy